LOW RESISTANCE JUNCTIONS IN CRAYFISH

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چکیده

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Low Resistance Junctions in Crayfish

The ultrastructure of low resistance junctions between segments of lateral giant fibers in crayfish is studied in sections from specimens fixed either by conventional methods or by glutaraldehyde-H(2)O(2) or by glutaraldehyde-lanthanum. Cross sections through junctions fixed by conventional glutaraldehyde display the usual trilaminar profile of two parallel membranes separated by a narrow gap. ...

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Low resistance junctions in crayfish. Structural changes with functional uncoupling

Electrical uncoupling of crayfish septate lateral giant axons is paralleled by structural changes in the gap junctions. The changes are characterized by a tighter aggregation of the intramembrane particles and a decrease in the overall width of the junction and the thickness of the gap. Preliminary measurements indicate also a decrease in particle diameter. The uncoupling is produced by in vitr...

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LOW RESISTANCE JUNCTIONS IN CRAYFISH I. Two Arrays of Globules in Junctional Membranes

The ultrastructure of low resistance junctions between segments of lateral giant fibers in crayfish is studied in sections from specimens fixed either by conventional methods or by glutaraldehyde-H202 or by glutaraldehyde-lanthanum. Cross sections through junctions fixed by conventional glutaraldehyde display the usual trilaminar profile of two parallel membranes separated by a narrow gap . Mos...

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LOW RESISTANCE JUNCTIONS IN CRAYFISH II. Structural Details and Further Evidence for Intercellular Channels by Freeze-Fracture and Negative Staining

Low resistance junctions between axons of crayfish ganglia are studied by freeze-fracture and negative staining . In freeze-fracture, fracture planes that go through a junctional membrane expose two faces, both internal, called face A and face B . Face A belongs to the internal membrane leaflet and faces the gap . Face B belongs to the external membrane leaflet and faces the axoplasm. Face A di...

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Gap and low-resistance junctions between cells in culture.

In vitro cultured cells are connected by different membrane junctions which may serve as a matrix for the monolayer, but which may also enable intercellu­ lar communication. Some of these intercellular infor­ mation exchanges may be detected by ionic coupling between the cells through so-called “low-resistance junctions” . Gilula and cow orkers1, for instance, have shown that metabolic cooperat...

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ژورنال

عنوان ژورنال: Journal of Cell Biology

سال: 1973

ISSN: 1540-8140,0021-9525

DOI: 10.1083/jcb.57.1.54